Article
High sensitivity detection and quantitation of DNA copy number and single nucleotide variants with single color droplet digital PCR.
Analytical chemistry - 4 Mar 2014
Miotke Laura, Lau Billy T, Rumma Rowza T, Ji Hanlee P
Abstract excerpt
In this study, we present a highly customizable method for quantifying copy number and point mutations utilizing a single-color, droplet digital PCR platform. Droplet digital polymerase chain reaction (ddPCR) is rapidly replacing real-time quantitative PCR (qRT-PCR) as an efficient method of independent DNA quantification. Compared to quantative PCR, ddPCR eliminates the needs for traditional standards; instead,...
Topics
- Base Sequence
- DNA Copy Number Variations
- DNA Primers
- Polymorphism, Single Nucleotide