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Article

High sensitivity detection and quantitation of DNA copy number and single nucleotide variants with single color droplet digital PCR.

Analytical chemistry - 4 Mar 2014

Miotke Laura, Lau Billy T, Rumma Rowza T, Ji Hanlee P

Abstract excerpt

In this study, we present a highly customizable method for quantifying copy number and point mutations utilizing a single-color, droplet digital PCR platform. Droplet digital polymerase chain reaction (ddPCR) is rapidly replacing real-time quantitative PCR (qRT-PCR) as an efficient method of independent DNA quantification. Compared to quantative PCR, ddPCR eliminates the needs for traditional standards; instead,...

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Identifiers

PMID: 24483992
DOI: 10.1021/ac403843j
PMCID: PMC3982983

Genes

BRAF
FLT3

Variants

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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