Article
Competitive allele-specific TaqMan PCR (Cast-PCR) is a sensitive, specific and fast method for BRAF V600 mutation detection in Melanoma patients.
Scientific reports - 22 Dec 2015
Barbano Raffaela, Pasculli Barbara, Coco Michelina, Fontana Andrea, Copetti Massimiliano, Rendina Michelina, Valori Vanna Maria, Graziano Paolo, Maiello Evaristo, Fazio Vito Michele, Parrella Paola
Abstract excerpt
BRAF codon 600 mutation testing of melanoma patients is mandatory for the choice of the most appropriate therapy in the clinical setting. Competitive allele specific TaqMan PCR (Cast-PCR) technology allows not only the selective amplification of minor alleles, but it also blocks the amplification of non-mutant allele. We genotyped codon 600 of the BRAF gene in 54 patients' samples by Cast-PCR and bidirectional...
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