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Article

Allele specific Taqman-based real-time PCR assay to quantify circulating BRAFV600E mutated DNA in plasma of melanoma patients.

Clinica chimica acta; international journal of clinical chemistry - 6 Sept 2010

Pinzani Pamela, Salvianti Francesca, Cascella Roberta, Massi Daniela, De Giorgi Vincenzo, Pazzagli Mario, Orlando Claudio

Abstract excerpt

BACKGROUND: BRAF is the most frequently mutated oncogene in melanoma with BRAF(V600E) mutation accounting for 92% of all BRAF variants. As this event occurs early in melanoma progression, the quantification of BRAF-mutated alleles in plasma may represent a useful biomarker for noninvasive diagnosis and prediction of response to therapy. METHODS: We propose an assay based on the use of a locked nucleic acid probe...

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PMID: 20576522
DOI: 10.1016/j.cca.2010.05.024

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BRAF

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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