Article
A Complex of Pyrosequencing-Based Methods for Detection of Somatic Mutations in Codons 600 and 601 of the BRAF gene.
Sovremennye tekhnologii v meditsine - 1 Jan 2022
Dribnokhodova O P, Esman A S, Korchagin V I, Bukharina A Yu, Dunaeva E A, Leshkina G V, Borisova E V, Voiciehovskaya Ya A, Daoud A I, Khlyavich V N, Mironov K O
Abstract excerpt
The aim of the study is to develop methods for the differentiation of mutations in the BRAF codon 600 and to increase the sensitivity of the K601E mutation detection. Materials and Methods: The nucleotide sequence of the BRAF codons 592-602 was identified using the PyroMark Q24 genetic analysis system. The mutations search in codon 600 was conducted using the 600-S primer in line with the following order of...
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