Article
Targeted gene disruption by use of CRISPR/Cas9 ribonucleoprotein complexes in the water flea Daphnia pulex.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Jun 2018
Hiruta Chizue, Kakui Keiichi, Tollefsen Knut E, Iguchi Taisen
Abstract excerpt
The microcrustacean Daphnia pulex is an important model for environmental, ecological, evolutionary and developmental genomics because its adaptive life history displays plasticity in response to environmental changes. Even though the whole-genome sequence is available and omics data have actively accumulated for this species, the available tools for analyzing gene function have thus far been limited to RNAi (RNA...
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