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Article

CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna.

PloS one - 1 Jan 2017

Kumagai Hitoshi, Nakanishi Takashi, Matsuura Tomoaki, Kato Yasuhiko, Watanabe Hajime

Abstract excerpt

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system (Cas) is widely used for mediating the knock-in of foreign DNA into the genomes of various organisms. Here, we report a process of CRISPR/Cas-mediated knock-in via non-homologous end joining by the direct injection of Cas9/gRNA ribonucleoproteins (RNPs) in the crustacean Daphnia magna, which is a model organism for...

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Identifiers

PMID: 29045453
DOI: 10.1371/journal.pone.0186112
PMCID: PMC5646780

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Source receipt: pubmed, retrieved 7 Sept 2026.

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