Article
CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna.
PloS one - 1 Jan 2017
Kumagai Hitoshi, Nakanishi Takashi, Matsuura Tomoaki, Kato Yasuhiko, Watanabe Hajime
Abstract excerpt
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system (Cas) is widely used for mediating the knock-in of foreign DNA into the genomes of various organisms. Here, we report a process of CRISPR/Cas-mediated knock-in via non-homologous end joining by the dir...
Topics
- Animals
- Animals, Genetically Modified
- Base Sequence
- CRISPR-Cas Systems
- DNA End-Joining Repair
- Daphnia
- Gene Knock-In Techniques
- Gene Knockout Techniques
- Genes, Reporter
- Genetic Loci
- Germ Cells
- Phenotype
- Plasmids
- Ribonucleoproteins
- Transgenes