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Article

Structural requirements for protein-catalyzed annealing of U4 and U6 RNAs during di-snRNP assembly.

Nucleic acids research - 18 Feb 2016

Didychuk Allison L, Montemayor Eric J, Brow David A, Butcher Samuel E

Abstract excerpt

Base-pairing of U4 and U6 snRNAs during di-snRNP assembly requires large-scale remodeling of RNA structure that is chaperoned by the U6 snRNP protein Prp24. We investigated the mechanism of U4/U6 annealing in vitro using an assay that enables visualization of ribonucleoprotein complexes and faithfully recapitulates known in vivo determinants for the process. We find that annealing, but not U6 RNA binding, is...

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Identifiers

PMID: 26673715
DOI: 10.1093/nar/gkv1374
PMCID: PMC4756825

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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