Article
Target selection by natural and redesigned PUF proteins.
Proceedings of the National Academy of Sciences of the United States of America - 29 Dec 2015
Porter Douglas F, Koh Yvonne Y, VanVeller Brett, Raines Ronald T, Wickens Marvin
Abstract excerpt
Pumilio/fem-3 mRNA binding factor (PUF) proteins bind RNA with sequence specificity and modularity, and have become exemplary scaffolds in the reengineering of new RNA specificities. Here, we report the in vivo RNA binding sites of wild-type (WT) and reengineered forms of the PUF protein Saccharomyces cerevisiae Puf2p across the transcriptome. Puf2p defines an ancient protein family present throughout fungi, with...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.