Article
A universal code for RNA recognition by PUF proteins.
Nature chemical biology - 15 May 2011
Filipovska Aleksandra, Razif Muhammad F M, Nygård Karoline K A, Rackham Oliver
Abstract excerpt
The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of diverse sequence and...
Topics
- Cytosine
- Electrophoretic Mobility Shift Assay
- Humans
- Mutation
- Protein Engineering
- RNA
- RNA-Binding Proteins
- Repetitive Sequences, Amino Acid
- Saccharomyces cerevisiae
- Two-Hybrid System Techniques