Article
A three-hybrid screen identifies mRNAs controlled by a regulatory protein.
RNA (New York, N.Y.) - 1 Aug 2006
Seay Daniel, Hook Brad, Evans Katie, Wickens Marvin
Abstract excerpt
RNA-protein interactions are important in many biological contexts. Identification of the networks that connect regulatory proteins to one another and to the mRNAs they control is a critical need. Here, we use a yeast three-hybrid screening approach to identify RNAs that bind a known RNA regulatory protein, the Saccharomyces cerevisiae PUF protein, Mpt5p. The assay selects RNAs that bind in vivo using simple...
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