Article
Divergent RNA binding specificity of yeast Puf2p.
RNA (New York, N.Y.) - 1 Aug 2011
Yosefzon Yahav, Koh Yvonne Y, Chritton Jacqueline J, Lande Avigail, Leibovich Limor, Barziv Lavi, Petzold Christine, Yakhini Zohar, Mandel-Gutfreund Yael, Wickens Marvin, Arava Yoav
Abstract excerpt
PUF proteins bind mRNAs and regulate their translation, stability, and localization. Each PUF protein binds a selective group of mRNAs, enabling their coordinate control. We focus here on the specificity of Puf2p and Puf1p of Saccharomyces cerevisiae, which copurify with overlapping groups of mRNAs. We applied an RNA-adapted version of the DRIM algorithm to identify putative binding sequences for both proteins....
Topics
- Base Sequence
- Binding Sites
- Mutation
- Protein Binding
- RNA, Messenger
- RNA-Binding Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Substrate Specificity