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Article

Engineering RNA sequence specificity of Pumilio repeats.

Proceedings of the National Academy of Sciences of the United States of America - 12 Sept 2006

Cheong Cheom-Gil, Hall Traci M Tanaka

Abstract excerpt

Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A "code" for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here, we report the creation of seven soluble mutant proteins with predictably altered sequence...

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Identifiers

PMID: 16954190
DOI: 10.1073/pnas.0606294103
PMCID: PMC1564246

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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