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Article

Tuneable endogenous mammalian target complementation via multiplexed plasmid-based recombineering.

Scientific reports - 27 Nov 2015

Beltran-Sastre Violeta, Benisty Hannah, Burnier Julia, Berger Imre, Serrano Luis, Kiel Christina

Abstract excerpt

Understanding the quantitative functional consequences of human disease mutations requires silencing of endogenous genes and expression of mutants at close to physiological levels. Changing protein levels above or below these levels is also important for system perturbation and modelling. Fast design optimization demands flexible interchangeable cassettes for endogenous gene silencing and tuneable expression....

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Source

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Identifiers

PMID: 26612112
DOI: 10.1038/srep17432
PMCID: PMC4661934

Genes

BRAF
HRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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