Article
Replacement of normal with mutant alleles in the genome of normal human cells unveils mutation-specific drug responses.
Proceedings of the National Academy of Sciences of the United States of America - 30 Dec 2008
Di Nicolantonio Federica, Arena Sabrina, Gallicchio Margherita, Zecchin Davide, Martini Miriam, Flonta Simona Emilia, Stella Giulia Maria, Lamba Simona, Cancelliere Carlotta, Russo Mariangela, Geuna Massimo, Appendino Giovanni, Fantozzi Roberto, Medico Enzo, Bardelli Alberto
Abstract excerpt
Mutations in oncogenes and tumor suppressor genes are responsible for tumorigenesis and represent favored therapeutic targets in oncology. We exploited homologous recombination to knock-in individual cancer mutations in the genome of nontransformed human cells. Sequential introduction of multiple mutations was also achieved, demonstrating the potential of this strategy to construct tumor progression models....
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