Article
Development of a highly resolutive method, using a double quadruplex tetra-primer-ARMS-PCR coupled with capillary electrophoresis to study CD40LG polymorphisms.
Molecular and cellular probes - 1 Dec 2015
Aloui Chaker, Sut Caroline, Cognasse Fabrice, Granados Viviana, Hassine Mohsen, Chakroun Tahar, Garraud Olivier, Laradi Sandrine
Abstract excerpt
Polymorphisms in the CD40 ligand gene (CD40LG) are associated with various immunological disorders such as tumors, autoimmune and infectious diseases. The aim of this study was to develop a highly optimized double quadruplex tetra-primer amplification refractory mutation system PCR (double quadruplex T-ARMS-PCR) coupled with capillary electrophoresis to allow genotyping of eight relevant candidate CD40LG SNPs and...
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