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Tetra-primer ARMS-PCR combined with GoldMag lateral flow assay for genotyping: simultaneous visual detection of both alleles.

Nanoscale - 14 May 2020

Zhang Sinong, Cai Yu, Zhang Jiaxing, Liu Xiaonan, He Lihua, Cheng Long, Hua Kai, Hui Wenli, Zhu Juanli, Wan Yinsheng, Cui Yali

Abstract excerpt

Rapid and simple detection of single nucleotide polymorphism (SNP) is vital for individualized diagnosis and eventual treatment in the current clinical setting. In this study, we developed a tetra-primer ARMS-PCR combined lateral flow assay (T-ARMS-PCR-LFA) method for simultaneous visual detection of two alleles. By using four primers labeled with digoxin, biotin and Cy5 separately in one PCR reaction, the...

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Alleles
Antibodies
Digoxin
DNA
DNA Primers

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Methylenetetrahydrofolate Reductase (NADPH2)

Polymerase Chain Reaction

Polymorphism, Single Nucleotide

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PMID: 32350488
DOI: 10.1039/d0nr00360c

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