Development and validation of a tetra-primer ARMS-PCR assay for genotyping the MTHFR (rs1801131) c.1286 A > C (p.Glu429Ala) polymorphism: a comparative study with KASP.

doi:10.1007/s11033-026-11930-8

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Development and validation of a tetra-primer ARMS-PCR assay for genotyping the MTHFR (rs1801131) c.1286 A > C (p.Glu429Ala) polymorphism: a comparative study with KASP.

Molecular biology reports - 16 May 2026

Alqosaibi Amany, Rasheed Akram Husain Rehman Syed, Tombuloglu Huseyin, Altwayan Reham, Alhusil Abdulrahman, Awadh Taghreed, Ozdemir Mehmet

Abstract excerpt

BACKGROUND: The tetra-primer amplification refractory mutation system (T-ARMS) PCR assay is a cost-effective and rapid method for SNP genotyping. The methylenetetrahydrofolate reductase (MTHFR) c.1286 A > C (rs1801131) polymorphism can influence MTHFR enzyme activity and homocysteine levels. While its independent clinical impact remains a subject of debate, it is frequently screened in thrombophilia panels,...

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PMID: 42142182
DOI: 10.1007/s11033-026-11930-8

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