Article
Guidelines for the tetra-primer ARMS-PCR technique development.
Molecular biotechnology - 1 Jul 2014
Medrano Ruan Felipe Vieira, de Oliveira Camila Andréa
Abstract excerpt
The tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR) reaction is a simple and economical method to genotype single-nucleotide polymorphisms (SNPs). It uses four primers in a single PCR and is followed just by gel electrophoresis. However, the optimization step can be very hardworking and time-consuming. Hence, we propose to demonstrate and discuss critical steps for its...
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