Article
Multiplex allele-specific amplification from whole blood for detecting multiple polymorphisms simultaneously.
Genetic testing and molecular biomarkers - 1 Jan 2013
Zhu Jianjie, Chen Lanxin, Mao Yong, Zhou Huan, Li Rui, Wang Weipeng
Abstract excerpt
Allele-specific amplification on the basis of polymerase chain reaction (PCR) has been widely used for single-nucleotide polymorphism (SNP) genotyping. However, the extraction of PCR-compatible genomic DNA from whole blood is usually required. This process is complicated and tedious, and is prone to cause cross-contamination between samples. To facilitate direct PCR amplification from whole blood without the...
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