Article
A novel multiplex tetra-primer ARMS-PCR for the simultaneous genotyping of six single nucleotide polymorphisms associated with female cancers.
PloS one - 1 Jan 2013
Zhang Chen, Liu Ying, Ring Brian Z, Nie Kai, Yang Mengjie, Wang Miao, Shen Hongwei, Wu Xiyang, Ma Xuejun
Abstract excerpt
BACKGROUND: The tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is a fast and economical means of assaying SNP's, requiring only PCR amplification and subsequent electrophoresis for the determination of genotypes. To improve the throughput and efficiency of T-ARMS-PCR, we combined T-ARMS-PCR with a chimeric primer-based temperature switch PCR (TSP) strategy, and used capillary...
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