Article
Development and validation of CALR mutation testing for clinical diagnosis.
American journal of clinical pathology - 1 Nov 2015
Maier Cheryl L, Fisher Kevin E, Jones Heather H, Hill Charles E, Mann Karen P, Zhang Linsheng
Abstract excerpt
OBJECTIVES: To validate a diagnostic assay for detecting CALR mutations in the clinical setting. METHODS: Traditional polymerase chain reaction (PCR) was performed on DNA previously extracted from 60 specimens (30 bone marrow aspirates [BMAs] and 30 peripheral blood [PB] samples) from 55 patients. Nearly all reported CALR mutations are insertions or deletions in exon 9. Therefore, we performed amplicon sizing by...
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