Article
[Detection of JAK2V617F and CALR Gene Mutations by Multiplex of Patients with Myeloproliferative Neoplasms PCR-Capillary Electrophoresis].
Zhongguo shi yan xue ye xue za zhi - 1 Dec 2020
Yuan Jian-Long, Shi Ying-Xu, DU Hua, Wang Ying-Jun, Zhao Zi-Ling, Li Ge, Han Yan-Qiu
Abstract excerpt
OBJECTIVE: To evaluate the proformance of multiplex PCR and capillary electrophoresis(MPCE) in the detection of JAK2V617F and CALR mutation in myeloproliferative neoplasms(MPN). METHODS: The specificity primers of JAK2617F gene mutation and the primers of CALR gene were designed at the same time. The JAK2V617F and CALR gene primers were labeled with Cy5 fluorescence, all the primers were mixed in one tube for...
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