Article
Gene essentiality and synthetic lethality in haploid human cells.
Science (New York, N.Y.) - 27 Nov 2015
Blomen Vincent A, Májek Peter, Jae Lucas T, Bigenzahn Johannes W, Nieuwenhuis Joppe, Staring Jacqueline, Sacco Roberto, van Diemen Ferdy R, Olk Nadine, Stukalov Alexey, Marceau Caleb, Janssen Hans, Carette Jan E, Bennett Keiryn L, Colinge Jacques, Superti-Furga Giulio, Brummelkamp Thijn R
Abstract excerpt
Although the genes essential for life have been identified in less complex model organisms, their elucidation in human cells has been hindered by technical barriers. We used extensive mutagenesis in haploid human cells to identify approximately 2000 genes required for optimal fitness under culture conditions. To study the principles of genetic interactions in human cells, we created a synthetic lethality network...
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