Identification of key residues in Debaryomyces hansenii carbonyl reductase for highly productive preparation of (S)-aryl halohydrins.

Abstract excerpt

Key residues of Debaryomyces hansenii carbonyl reductase in the determination of the reducing activity towards aryl haloketones were identified through combinatorial mutation of conserved residues. This study provides a green and efficient biocatalyst for the synthesis of (S)-aryl halohydrins.

Topics

• Alcohol Oxidoreductases
• Catalysis
• Chlorohydrins
• Fungal Proteins
• Models, Molecular
• Mutation
• Saccharomycetales