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Article

Overcoming the concentration-enantioselectivity trade-off in haloalcohol dehalogenase for high-titer (R)-epichlorohydrin production.

International journal of biological macromolecules - 1 Apr 2026

Wu Kuijun, Lai Zhuofan, Xu Yan, Wu Tao, Mu Xiaoqing

Abstract excerpt

The biocatalytic production of enantiopure (R)-epichlorohydrin (ECH) using haloalcohol dehalogenases (HHDHs) is often limited by a concentration-dependent erosion of enantioselectivity, a phenomenon primarily driven by mutual inhibition between substrate enantiomers. This work reports the engineering of the C-type HHDH from Agrobacterium radiobacter (HheC) through structure-guided rational modification to...

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PMID: 41926861
DOI: 10.1016/j.ijbiomac.2026.151790

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