Article
Exploring the thermostable properties of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by a combinatorial directed evolution strategy.
Applied microbiology and biotechnology - 1 Apr 2017
Wu Zhiyun, Deng Wenfeng, Tong Yapei, Liao Qian, Xin Dongmin, Yu Huashun, Feng Juan, Tang Lixia
Abstract excerpt
As a crucial factor for biocatalysts, protein thermostability often arises from a combination of factors that are often difficult to rationalize. In this work, the thermostable nature of halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC) was systematically explored using a combinatorial directed evolution approach. For this, a mutagenesis library of HheC mutants was first constructed using...
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