Article
Substrate specificity engineering of leucine dehydrogenase for efficient synthesis of aromatic L-homophenylalanine.
International journal of biological macromolecules - 1 May 2026
Zhao Lu, Xing Wenyin, Zhang Wenhe, Liu Qi, Tang Lichun, Chen Juanjie, Gao Xiao, Jia Xian, You Song
Abstract excerpt
Leucine dehydrogenase (LeuDH)-catalyzed asymmetric reductive amination of α-keto acids represents a promising approach for synthesizing L-amino acids. However, the stringent substrate specificity restricts its application primarily to aliphatic amino acid synthesis. Herein, we conducted substrate-specific engineering of Bacillus subtilis leucine dehydrogenase (BsLeuDH) for bulky aromatic L-homophenylalanine...
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