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Article

A high-throughput screening assay for the directed evolution-guided discovery of halohydrin dehalogenase mutants for epoxide ring-opening reaction.

Journal of biotechnology - 10 Mar 2020

Gul Ijaz, Fantaye Bogale Tadesse, Deng Jiao, Wang Le, Feng Juan, Tang Lixia

Abstract excerpt

Halohydrin dehalogenases (HHDHs) are valuable biocatalysts involved in the synthesis of β-substituted alcohols via their nucleophile-mediated ring-opening activity. To use directed evolution to unleash the latent potential of HHDHs for the synthesis of β-substituted alcohols, we report a high-throughput assay for screening HHDHs mutagenesis libraries. The assay is performed in a 96-well microtiter plate format...

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Identifiers

PMID: 32067982
DOI: 10.1016/j.jbiotec.2020.02.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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