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Article

Substrate expansion of Geotrichum candidum alcohol dehydrogenase towards diaryl ketones by mutation.

Applied microbiology and biotechnology - 27 Dec 2024

Tang Zhongyao, Takagi Yuuki, Koesoema Afifa Ayu, Matsuda Tomoko

Abstract excerpt

Chiral diaryl alcohols, such as (4-chlorophenyl)(pyridin-2-yl)methanol, are important intermediates for pharmaceutical synthesis. However, using alcohol dehydrogenases (ADHs) in the asymmetric reduction of diaryl ketones to produce the corresponding alcohols is challenging due to steric hindrance in the substrate binding pockets of the enzymes. In this study, the steric hindrance of the ADH from Geotrichum...

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Identifiers

PMID: 39729095
DOI: 10.1007/s00253-024-13375-0
PMCID: PMC11680648

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