Article
Conversion of aminodeoxychorismate synthase into anthranilate synthase with Janus mutations: mechanism of pyruvate elimination catalyzed by chorismate enzymes.
Biochemistry - 14 Apr 2015
Culbertson Justin E, Chung Dong hee, Ziebart Kristin T, Espiritu Eduardo, Toney Michael D
Abstract excerpt
The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically similar. The first catalytic step, amination at C2, is common between them, but AS additionally...
Topics
- Anthranilate Synthase
- Computational Biology
- Escherichia coli
- Kinetics
- Lyases
- Molecular Dynamics Simulation
- Mutation
- Protein Conformation
- Pyruvates
- Thermodynamics
- Transaminases