Article
[Construction of T-vectors for the direct, unidirectional cloning and analysis of PCR-amplified promoters].
Molekuliarnaia biologiia - 1 Jan 2000
Wang B L, Li X X, Zheng F, Liu R, Quan J X, Jia H W, Liang H, Deng T, Guo S Y, Guo G, Zhang J Y, Qiu M C
Abstract excerpt
The amplification and cloning of promoters are regularly employed procedures to study the mechanism of gene regulation. In the present study we developed a method to construct T-vectors used for the direct and unidirectional cloning and analysis of promoters. These so-called T-vectors pEGFP-T and pGL3-T were derived from their parent promoterless vectors pEGFP-1 and pGL3-basic, respectively. To construct the...
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