Article
[Transforming effect of PDGFRA gene mutant on the cell function in gastrointestinal stromal tumor].
Zhonghua zhong liu za zhi [Chinese journal of oncology] - 1 Jul 2009
Yang Lei, Bai Chen-Guang, Hou Xiao-Wei, Liu Xiao-Hong, Ma Da-Lie
Abstract excerpt
OBJECTIVE: To explore the effect of malignant transformation of the L839P, a new mutation site of the PDGFRA gene, on the pathogenesis of gastrointestinal stromal tumors. METHODS: All recombinant plasmids were stably transfected into CHO cells by liposomes. Western blotting was used to detect the expression of PDGFRA protein. The cell growth curve was plotted by cell counting. Flow cytometry was used to detect...
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