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Article

Rapid mutagenesis-based analysis of phosphorylation sites in mitogen-activated protein kinase substrates.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Eschen-Lippold Lennart, Bauer Nicole, Löhr Julia, Palm-Forster Mieder A T, Lee Justin

Abstract excerpt

In eukaryotes, mitogen-activated protein kinases (MAPKs) are one of the best studied pathways for posttranslational modification-mediated regulation of protein functions. Here, we describe a rapid in vitro method to screen potential protein phosphorylation sites targeted by MAPKs. The method is based on PCR-mediated mutagenesis together with a type IIs restriction digest. Screening for the successfully mutated...

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Source

Identifiers

PMID: 24908129
DOI: 10.1007/978-1-4939-0922-3_15

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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