Article
[Effect of Plk1 330/597 serine phosphorylated mutant on cytokinesis during mitosis].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Oct 2012
Cheng Li, Chen Li-jian, An Ran, Du Jian
Abstract excerpt
AIM: To explore the locations of pseudo-phosphorylated mutant (Plk1(S330/597D);) and non-phosphorylatable mutant (Plk1(S330/597A);) of Plk1 (residues 330 and 597 serine) in HeLa cells and the effect on mitosis. METHODS: We transfected wide-type Plk1-GFP, Plk1(S330/597D);-GFP and Plk1(S330/597A);-GFP into HeLa cells, respectively, and detected the locations of wide type and mutants in HeLa cells using...
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