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Article

Production of α1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning.

The Journal of reproduction and development - 16 Feb 2017

Gao Hanchao, Zhao Chengjiang, Xiang Xi, Li Yong, Zhao Yanli, Li Zesong, Pan Dengke, Dai Yifan, Hara Hidetaka, Cooper David K C, Cai Zhiming, Mou Lisha

Abstract excerpt

Gene-knockout pigs hold great promise as a solution to the shortage of organs from donor animals for xenotransplantation. Several groups have generated gene-knockout pigs via clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) and somatic cell nuclear transfer (SCNT). Herein, we adopted a simple and micromanipulator-free method, handmade cloning (HMC) instead of SCNT, to...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 27725344
DOI: 10.1262/jrd.2016-079
PMCID: PMC5320426

Genes

GGTA1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 3 Sept 2026.

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