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Article

PCR-based screening of targeted mutants for the fast and simultaneous identification of bacterial virulence factors.

BioTechniques - 1 Jul 2012

Henriques Ana, Carvalho Filipe, Pombinho Rita, Reis Olga, Sousa Sandra, Cabanes Didier

Abstract excerpt

Understanding the strategies used by pathogens to infect, survive, and proliferate in their hosts requires the identification of virulence factors. We developed PCR-based screening of targeted mutants to facilitate quick, simultaneous detection of multiple novel bacterial virulence genes. Based on direct PCR screening of pooled targeted mutants, this approach provides a fast and sensitive measure of virulence...

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Identifiers

PMID: 26307257
DOI: 10.2144/000113906

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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