Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes.

doi:10.1007/978-1-0716-0982-8_14

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Article

Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Parsons Cameron, Azizoglu Reha, Elhanafi Driss, Kathariou Sophia

Abstract excerpt

Genes that play a role in stress response mechanisms and other phenotypes of Listeria monocytogenes can be identified by construction and screening of mutant libraries. In this chapter, we describe the construction and screening of mutant libraries of L. monocytogenes using the plasmid pMC38, carrying a mariner-based transposon system (TC1/mariner) and constructed by Cao et al. (Appl Environ Microbiol...

Topics

Electroporation
Genetic Vectors
Humans
Listeria monocytogenes
Listeriosis
Mutagenesis, Insertional
Mutation
Plasmids
Polymerase Chain Reaction
Retroelements

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Open in PubMed

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PMID: 32975775
DOI: 10.1007/978-1-0716-0982-8_14

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