Article
Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread.
Infection and immunity - 1 Nov 1990
Sun A N, Camilli A, Portnoy D A
Abstract excerpt
To dissect the regulatory and structural requirements for Listeria monocytogenes intracellular growth and cell-to-cell spread, we designed a protocol based on transposon mutagenesis and the isolation of mutants which form small plaques in monolayers of mouse L2 cell fibroblasts. Two different transposable elements were used to generate libraries of insertion mutants: Tn916 and a derivative of Tn917-lac,...
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