Article
Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread.
Infection and immunity - 1 Nov 1990
Sun A N, Camilli A, Portnoy D A
Abstract excerpt
To dissect the regulatory and structural requirements for Listeria monocytogenes intracellular growth and cell-to-cell spread, we designed a protocol based on transposon mutagenesis and the isolation of mutants which form small plaques in monolayers of mouse L2 cell fibroblasts. Two different tra...
Topics
- Actins
- Base Sequence
- Cells, Cultured
- DNA Transposable Elements
- Egg Yolk
- Hemolysis
- Humans
- Listeria monocytogenes
- Molecular Sequence Data
- Mutation
- Open Reading Frames
- Phospholipases