Article
Discriminating a Single Nucleotide Difference for Enhanced miRNA Detection Using Tunable Graphene and Oligonucleotide Nanodevices.
Langmuir : the ACS journal of surfaces and colloids - 15 Sept 2015
Robertson Neil M, Hizir Mustafa Salih, Balcioglu Mustafa, Wang Rui, Yavuz Mustafa Selman, Yumak Hasan, Ozturk Birol, Sheng Jia, Yigit Mehmet V
Abstract excerpt
In this study we have reported our efforts to address some of the challenges in the detection of miRNAs using water-soluble graphene oxide and DNA nanoassemblies. Purposefully inserting mismatches at specific positions in our DNA (probe) strands shows increasing specificity against our target miRNA, miR-10b, over miR-10a which varies by only a single nucleotide. This increased specificity came at a loss of signal...
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