New insights into the molecular mechanism of the Rab GTPase Sec4p activation.

Abstract excerpt

BACKGROUND: Sec4p is a small monomeric Ras-related GTP-binding protein (23 kDa) that regulates polarized exocytosis in S. cerevisiae. In this study we examine the structural effects of a conserved serine residue in the P-loop corresponding to G12 in Ras. RESULTS: We show that the Sec4p residue serine 29 forms a hydrogen bond with the nucleotide. Mutations of this residue have a different impact than equivalent...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Crystallography, X-Ray
• Share to Guanine Nucleotide Exchange Factors
• Share to Protein Multimerization