Article
Resurrection of a functional phosphatidylinositol transfer protein from a pseudo-Sec14 scaffold by directed evolution.
Molecular biology of the cell - 15 Mar 2011
Schaaf Gabriel, Dynowski Marek, Mousley Carl J, Shah Sweety D, Yuan Peihua, Winklbauer Eva M, de Campos Marília K F, Trettin Kyle, Quinones Mary-Chely, Smirnova Tatyana I, Yanagisawa Lora L, Ortlund Eric A, Bankaitis Vytas A
Abstract excerpt
Sec14-superfamily proteins integrate the lipid metabolome with phosphoinositide synthesis and signaling via primed presentation of phosphatidylinositol (PtdIns) to PtdIns kinases. Sec14 action as a PtdIns-presentation scaffold requires heterotypic exchange of phosphatidylcholine (PtdCho) for PtdIns, or vice versa, in a poorly understood progression of regulated conformational transitions. We identify mutations...
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