Article
Hydrolysis of GTP by Sec4 protein plays an important role in vesicular transport and is stimulated by a GTPase-activating protein in Saccharomyces cerevisiae.
Molecular and cellular biology - 1 May 1992
Walworth N C, Brennwald P, Kabcenell A K, Garrett M, Novick P
Abstract excerpt
Sec4, a GTP-binding protein of the ras superfamily, is required for exocytosis in the budding yeast Saccharomyces cerevisiae. To test the role of GTP hydrolysis in Sec4 function, we constructed a mutation, Q-79----L, analogous to the oncogenic mutation of Q-61----L in Ras, in a region of Sec4 pre...
Topics
- Amino Acid Sequence
- Binding, Competitive
- Cell Fractionation
- Centrifugation, Density Gradient
- Cytoplasmic Granules
- Fungal Proteins
- GTP-Binding Proteins
- GTPase-Activating Proteins
- Genotype
- Guanosine 5'-O-(3-Thiotriphosphate)
- Guanosine Triphosphate
- Hydrolysis
- Kinetics