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Article

Identification of a functional hotspot on ubiquitin required for stimulation of methyltransferase activity on chromatin.

Proceedings of the National Academy of Sciences of the United States of America - 18 Aug 2015

Holt Matthew T, David Yael, Pollock Sam, Tang Zhanyun, Jeon Jongcheol, Kim Jaehoon, Roeder Robert G, Muir Tom W

Abstract excerpt

Ubiquitylation of histone H2B at lysine 120 (H2B-Ub) plays a critical role in transcriptional elongation, chromatin conformation, as well as the regulation of specific histone H3 methylations. Herein, we report a strategy for the site-specific chemical attachment of ubiquitin to preassembled nucleosomes. This allowed expedited structure-activity studies into how H2B-Ub regulates H3K79 methylation by the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26240340
DOI: 10.1073/pnas.1504483112
PMCID: PMC4547310

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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