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Article

Ubiquitin-Activated Interaction Traps (UBAITs) identify E3 ligase binding partners.

EMBO reports - 1 Dec 2015

O'Connor Hazel F, Lyon Nancy, Leung Justin W, Agarwal Poonam, Swaim Caleb D, Miller Kyle M, Huibregtse Jon M

Abstract excerpt

We describe a new class of reagents for identifying substrates, adaptors, and regulators of HECT and RING E3s. UBAITs (Ubiquitin-Activated Interaction Traps) are E3-ubiquitin fusion proteins and, in an E1- and E2-dependent manner, the C-terminal ubiquitin moiety forms an amide linkage to proteins that interact with the E3, enabling covalent co-purification of the E3 with partner proteins. We designed UBAITs for...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26508657
DOI: 10.15252/embr.201540620
PMCID: PMC4693525

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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