Article
Controlled enzymatic synthesis of natural-linkage, defined-length polyubiquitin chains using lysines with removable protecting groups.
Chemical communications (Cambridge, England) - 21 Feb 2011
Castañeda Carlos A, Liu Jia, Kashyap Tanuja R, Singh Rajesh K, Fushman David, Cropp T Ashton
Abstract excerpt
E2 enzymes catalyze the ATP-dependent polymerization of polyubiquitin chains which function as molecular signals in the regulation of numerous cellular processes. Here we present a method that uses genetically encoded unnatural amino acids to halt and re-start ubiquitin polymerization providing access to natural-linkage, precision-length ubiquitin chains that can be used for biochemical, structural, and dynamics...
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