Article
Epidermal growth factor receptor mutation status in cell-free DNA supernatant of bronchial washings and brushings.
Cancer cytopathology - 1 Oct 2015
Kawahara Akihiko, Fukumitsu Chihiro, Taira Tomoki, Abe Hideyuki, Takase Yorihiko, Murata Kazuya, Yamaguchi Tomohiko, Azuma Koichi, Ishii Hidenobu, Takamori Shinzo, Akiba Jun, Hoshino Tomoaki, Kage Masayoshi
Abstract excerpt
BACKGROUND: The aim of the current study was to examine whether it would be possible to detect epidermal growth factor receptor (EGFR) mutations in cytology cell-free DNA (ccfDNA) from the supernatant fluids of bronchial cytology samples. METHODS: This study investigated cell damage via immunostaining with a cleaved caspase 3 antibody and the quantity of cell-free DNA in supernatant fluid from 2 cancer cell...
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