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Article

Identification of a T7 RNA polymerase variant that permits the enzymatic synthesis of fully 2'-O-methyl-modified RNA.

Journal of biotechnology - 10 Sept 2013

Ibach Jenny, Dietrich Laura, Koopmans Kyra R M, Nöbel Nico, Skoupi Marc, Brakmann Susanne

Abstract excerpt

T7 RNA polymerase is an important biocatalyst that is used in diverse biotechnological applications such as in vitro transcription or protein expression. The enzyme displays high substrate specificity which is payed by significant limitations regarding incorporation of synthetic nucleotide analogs. Of specific interest is enzymatic synthesis of 2'-O-methyl-modified RNA as these nucleic acids exhibit improved...

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PMID: 23871655
DOI: 10.1016/j.jbiotec.2013.07.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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