RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes.

Abstract excerpt

DNA-dependent T7 RNA polymerase (T7 RNAP) is the most powerful tool for both gene expression and in vitro transcription. By using a Next Generation Sequencing (NGS) approach we have analyzed the polymorphism of a T7 RNAP-generated mRNA pool of the mboIIM2 gene. We find that the enzyme displays a relatively high level of template-dependent transcriptional infidelity. The nucleotide misincorporations and multiple...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Amino Acids
• Share to DNA-Directed RNA Polymerases
• Share to Epigenesis, Genetic