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Article

Rapid Screening of Gli2/3 Mutants Using the Flp-In System.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Niewiadomski Pawel, Rohatgi Rajat

Abstract excerpt

Gli2 and Gli3 respond to the Hedgehog (Hh) signal in mammals by undergoing posttranslational modifications and moving to the nucleus. The study of Gli proteins has been hampered by the fact that their overexpression in cells prevents their proper regulation. To address this issue, we have developed a method of rapid generation of stable cell lines expressing near-endogenous and approximately equal levels of...

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Identifiers

PMID: 26179044
DOI: 10.1007/978-1-4939-2772-2_11

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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