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Article

Validation of a locked nucleic acid based wild-type blocking PCR for the detection of EGFR exon 18/19 mutations.

Diagnostic pathology - 29 May 2015

Vliegen Liesbet, Dooms Christophe, De Kelver Wim, Verbeken Eric, Vansteenkiste Johan, Vandenberghe Peter

Abstract excerpt

BACKGROUND: Treatment decisions in advanced non-small cell lung cancer rely on accurate analysis of the EGFR mutation status in small tissue samples. Sanger sequencing of PCR products is unbiased and cheap, but its detection threshold requiring 20 % infiltration by malignant cells is not optimal. Commercial kits, based on quantitative real-time PCR have better detection limits and can detect a wide spectrum of...

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Identifiers

PMID: 26022577
DOI: 10.1186/s13000-015-0293-1
PMCID: PMC4448309

Genes

EGFR

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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